Suppose there is a mutation in the c subunit of atp synthase, such that the glutamate found in the middle of one of the membrane spanning helices is converted to a valine. what is likely to be the effect on atp synthesis and why? what are uncouplers? provide an example of when this might be useful. why is there a major and a minor groove in dna? how is the processivity of dna polymerase iii accomplished?