Biology, 23.11.2019 04:31 lesliegarza0805
Assume that a culture of e. coli was grown for approximately 50 generations in 15n (provided in the medium in the ammonium ion), which is a heavy isotope of nitrogen (14n). you extract the dna from the culture, and it has a density of 1.723 gm/cm3 (water = 1.00 gm/cm3). from the literature, you determine that dna containing only the common form of nitrogen, 14n, has a density of 1.700 gm/cm3. bacteria from the 15n culture were washed in buffer and transferred to 14n medium for one generation immediately after which the dna was extracted and its density determined.
what would be the expected density of the extracted dna?
what band(s) would be expected if subjected to ultracentrifugation through a cscl gradient?
(b) how would your answer to (a) above differ if replication occurred in a conservative fashion?
(c) assume that the extracted dna was completely denatured (separated into two single strands by heating at 90oc for 15 minutes), what would be the density (or densities) of the dna (s) in the heated extract?
Answers: 2
Biology, 21.06.2019 19:00
Can someone me with this and tell me what’s fracking and what does it have to do with carbon, carbon dioxide, or fossil fuels?
Answers: 2
Biology, 22.06.2019 03:30
Students in biology are studying the macromolecules of life. they used a calorimeter to determine the calories in various types of food. once the lab was completed, the students ate the left over food samples. monica commented that in just 6 or 7 "chews" of the saltine, it was gone; nothing but a sticky paste in her mouth. elaborate on what happened chemically while chewing the saltine. include the macromolecules present.
Answers: 1
Biology, 22.06.2019 05:00
What is the farthest destination to date for a human spaceflight mission?
Answers: 1
Biology, 22.06.2019 05:40
When new rock is added to an oceanic ridge, the magnetized strips on either side of the ridge are evidence of sea-floor spreading. this is because the rocks on the two sides of the ridge o are equal in width and in polarity are polar opposites oare magnetized vary in width but are equal in polarity
Answers: 3
Assume that a culture of e. coli was grown for approximately 50 generations in 15n (provided in the...
Mathematics, 28.08.2021 15:40
Social Studies, 28.08.2021 15:40
Physics, 28.08.2021 15:40
English, 28.08.2021 15:40
Mathematics, 28.08.2021 15:40
Mathematics, 28.08.2021 15:40
Mathematics, 28.08.2021 15:50
Business, 28.08.2021 15:50
Chemistry, 28.08.2021 15:50
Social Studies, 28.08.2021 15:50
Mathematics, 28.08.2021 15:50