Chromatography columns have a limited protein binding capacity during lab #4 you should not have ''overloaded'' the ni^+2 agarose column. yet you may have noticed that the w2 fraction fluoresced slightly. if you see a band in the western blot w2 lane, what does this data say about the physical protein structure of rgfp in that lane? give a possible mw for this band based upon the data (show your work)6. (4pts) if you did ''overloaded'' your ni^+2 agarose column with a gce sample that contained too much rgfp, would you expect to see a band in the w2 lane of the western blot? if so what is its mw? would you expect to see a band in the e2 lane? if so what is its mw? 7. (3pts) you develop the western blot, what mw size band in lane e3 are you expecting to observe? if you happen to see two bands - one at the expected mw and one lower band - what might you specifically conclude about the physical protein structure of the lower band?