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Biology, 09.11.2019 02:31 kenziepickup

Forward primer: 5'-agtctactcgtaaccggttacc-3' reverse primer: 5'-taaggcatcatggtaaccggtt-3' the two primers above have the melting temperatures between 55c-80c. what is the reason that these two primers will not bind effectively to the region of dna that we want to amplify?

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