When bacteria are inoculated into a new sterile nutrient broth, their numbers don't begin to increase immediately. instead, there is a lag phase that may last for an hour or even several days. why don't bacterial numbers increase immediately?
a. the bacteria have to establish a biofilm before their numbers can increase.
b. there are not enough nutrients for the bacteria to grow, and growth is delayed until there are some dead cells to cannibalize.
c. the medium contains inhibitors that prohibit rapid growth of the bacteria, and these must be inactivated before bacterial numbers will increase.
d. the bacteria must adjust to the nutrient content in the new medium, synthesizing necessary amino acids, growth factors, and enzymes.

Astudent from one of the research labs is having trouble preparing a slide for examination and photographing. the bacterial slide that he has brought to you was prepared using a commercially purchased stain. he has asked for your in determining what he is doing wrong so that he can change the lab protocols and continue on with his project. after examining the slide under oil immersion, you determine that no bacteria are present even though the student is able to show you the culture he used to make that slide that has visible growth in the liquid medium. which of the following statements does not explain the fact that there are no bacteria present on the student’s slide? by not allowing a glass slide to completely air dry before heat fixation, the flame will cause the surrounding water to boil and this will damage the bacterial cell. overheating during the fixation step boiled the water within the bacterial cells and resulted in the cells bursting. insufficient heating of the slide did not drive out the thin layer of water and this resulted in minimal bonding between the bacteria and the glass slide. rinsing with alcohol during the washing step stripped the bacteria off the glass slide. rinsing with alcohol during the washing step stripped the bacteria off the glass slide.