Bacteria classified as gram+contain many layers.
1. pseudomurein
2. phospholipids
3. cholesterol
4. peptidoglycan

4. Peptidoglycan

Explanation:

"Most bacteria can be broadly classified as Gram positive or Gram negative.

Gram positive bacteria have cell walls composed of thick layers of peptidoglycan.

Gram positive cells stain purple when subjected to a Gram stain procedure.

Gram negative bacteria have cell walls with a thin layer of peptidoglycan. The cell wall also includes an outer membrane with lipopolysaccharide (LPS) molecules attached."

Reference: Bailey, Regina. “Gram Positive vs. Gram Negative Bacteria.” ThoughtCo, ThoughtCo, 15 Oct. 2018

The correct answer is number 4. Peptidoblycan

Explanation:

  These bacteria, when performing the Gram staining technique, have a violet or dark blue stain. Gram-positive organisms are able to retain the violet dye due to the large amount of peptidoglycan in their cell wall. Cell walls of gram-positive organisms usually lack the peripheral membrane present in gram-negative bacteria.

  Peptidoglycan, also called murein, is a peptide-bound heteropolysaccharide present in the prokaryote cell wall. It is formed by two types of sugars (N-acetylmuramic acid and N-acetylglucosamine) and some amino acids.

4.) metal

"-the movement of carbon from the nonliving environment into living things and back to the environment

-carbon is the essential component of proteins, fats, and carbohydrates (which make up all organisms)

-carbon exists in air, water, and living organisms

-producers convert carbon dioxide in the atmosphere into carbohydrates during photosynthesis

-bacteria in the atmosphere cycles through the system in filtration units

-in cellular respiration, carbon is released back into the atmosphere as carbon dioxide

-some carbon is stored in limestone (forming one of the largest "carbon sinks" on earth)

-largest carbon reservoir on earth= limestone

-carbon stored in the bodies of organisms as fat, oils, or other molecules may be released into the soil or air when the organism dies

-this may form deposits of coal, oil, or natural gas (fossil fuels, which increased the atmospheric carbon dioxide)

-fossil fuels store carbon left over from bodies of organisms that died millions of years ago

-they are located in the surface of earth, deep within the earth and beneath the ocean floor."