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Biology, 07.10.2019 19:10 brittanysanders
An increasingly popular mechanism of gene knockout in bacterial cells is to use the crispr/cas9 system. the system uses a single-guide rna (sgrna) to direct the crispr associated protein 9 (cas9) endonuclease enzyme to a gene of interest. here, the cas9 protein will create a blunt, double-stranded break in the dna. the cas9 protein simply cuts, however, and does not directly cause a mutation to occur. by what mechanism does this system cause mutations?
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