The processivity of a dna polymerase depends on all of the following actions, except for
a. association of the dna polymerase with a sliding clamp (like eukaryotic pcna)
b. interaction between the thumb domain of dna polymerase and the dna
c. interaction between the minor groove of dna and the palm domain of the dna polymerase
d. loss of the hydrogen from the 3′-oh of the primer due to interaction with a divalent metal ion associated with the palm domain of the dna polymerase

Option (D).

Explanation:

Processivity of DNA polymerase may be defined as the number of nucleotides added in the single binding event. The synthesis phase of cell cycle is fully dependent on the processivity of DNA polymerase.

The processivity of DNA polymerase depends on the sliding clamp, domains of the DNA and the interaction of DNA with enzyme. Loss of hydrogen ion at 3'OH primer does not affect processivity but important for the nucleophile attack and also effect the catalytic property of enzyme.

Thus, the correct answer is option (D).

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