Using your knowledge of protein structure explain in detail the effect of exposing an enzyme to a ph outside of its optimal range. include the effect on both enzyme structure and functional

A change in pH can change the ionization of the side groups of basic and acidic amino acids of proteins., It is important to remember that these charges of are importance in stabilizing a protein secondary and tertiary structure and consequently affects its function. Changing these charges alters these interactions and disturbs protein loops such as beta-sheets and alpha-helices. This can change the structure of a proteins enzyme’s active site and cause it to fail to bind to a substrate or catalyze a reaction.

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explanation:

this protocol provides a detailed procedure for chromatin immunoprecipitation (chip) to study the dna-protein interactions.