If you were to use crispr to cleave dna at sites flanking a gene, and in this way produce a deletion of the gene, how many sgrnas would you need?

2

Explanation:

If the sequence that you want to delete is flanked by different sequences, you will need 2 sgRNAs: one to produce the cut at the 5´ side, and other complementary at the 3´ site.

However, in the unlikely case in which both of the flanking sequences are almost the same ( more than a 90% of identity)  or if it is a palindromic pattern, you can use the same sgRNA to guide the cuts.

Hope you luck!

c

explanation:

volcanoes (an event in the geosphere) release a large amount of particulate matter into the atmosphere. these particles serve as nuclei for the formation of water droplets (hydrosphere). rainfall (hydrosphere) often increases following an eruption, stimulating plant growth (biosphere).