Amutation that results in a single amino acid substitution in the active site of an enzyme a) may alter the ability of a noncompetitive inhibitor to bind to the enzyme. b) may alter the ability of an allosteric regulator to alter enzyme activity. c) may change the substrate specificity of the enzyme. d) may alter the ∆g for the reaction catalyzed by the enzyme.

The correct answer out of the four provided options is given by

option C). may change the substrate specificity of the enzyme.

Explanation:

This can be explained as substitution of a single amino acid in the active site of an enzyme will result in the alteration of activity in a protein as this substitution, in fact, has an affect on the assembly, folding or activity. An active site aids to bind the substrate to the enzyme. In case, it gets altered the specificity of the enzyme will be lost to a substrate and hence efficiency will be affected too

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