Explain why the inoculated plates are inverted during incubation.

Petri plates are incubated upside down to prevent contamination.

Explanation:

The plates are always incubated inverted or turned upside down on their covers during storage. This is done to prevent evaporation if plaque is stored for long periods, which can affect the growth efficiency of bacteria, or allow contamination by multiplying unwanted organisms such as mold.

Once the plaques have been filled with a damp suspension of bacteria, it should be allowed to evaporate shortly before overnight incubation. However, a moderate amount of moisture will still be present. If plaque is not inverted during incubation, bacteria will not be able to attach to the culture medium properly, which will either prevent them from growing and forming colonies properly or will encourage the growth of undesirable microorganisms.

Any of these results will invalidate the experiment. In addition, any condensation or moisture can cause streaking, which will make it difficult to select and analyze separate colonies. After bacteria form colonies, plaque is also stored upside down to maintain moisture levels.

correct answer: biotic and abiotic limitations

carrying capacity is defined as the total number of an organism who can be supported in a given area within the natural resources limit and without harming or degrading the natural, economic or the cultural environment present for the future generations.

carrying capacity is determined by the limiting factors, which are known to affect the natural resources available. it may include water, nutrients, minerals, space, light etc.

explanation:

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