A50-ml crude extract of skeletal muscle contains 32 mg of protein per ml. ten μl of the extract catalyzes a reaction at a rate of 0.14 μmol product formed per minute. the extract was fractionated by ammonium sulfate precipitation, and the fraction precipitating between 20% and 40% saturation was redissolved in10 ml. this solution contains 50 mg/ml protein. ten μl of this purifi ed fraction catalyzes the reaction at a rate of 0.65 μmol/min. (a) what is the degree of purifi cation (fold purifi cation)? (b) what is the percent yield of the enzyme recovered in the purifi ed fraction?
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A50-ml crude extract of skeletal muscle contains 32 mg of protein per ml. ten μl of the extract cata...
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Computers and Technology, 09.12.2019 18:31
