If threads are layered from top to bottom brown green red which layer will come into focus first if you are using the microscope properly

The most generally used magnification corresponding to the thread or band colors is as follows:  

The brown band is indicated by the magnification at 2 to 2.5x.  

The green band is indicated by the resolution of 16x to 20x.  

The red color corresponds to the magnification at 4x to 5x. When observing at a slide of three crossed threads, the brown appears on the bottom, which is followed by red in the middle and then the green at the top. Thus, the red layer will come first into focus if one is using the microscope properly.