Several species of leopard frogs are common throughout north america, where their ranges overlap. different species of leopard frogs are very similar in appearances, but the males of each species have a unique courtship call. how did speciation most likely occur in leopard frogs? a- reproductive isolation occured through behavioral isolation b- reproductive isolation occured through temporal isolation c- populations were separated through geographical isolation d- populations were separated through competition

the correct answer is A- Reproductive isolation occurred through behavioral isolation

A process of formation of a new species from an existing species is called speciation. New species may formed due to geographical or reproductive barriers which isolates the members of a population of a species to form a new species.

In the given question, speciation in In the leopard frogs occurred due to a particular or specific call given by males to the females. The courtship call lead to reproductive isolation due to change in behavior. Such type of behavioral change will help in recognizing mates of the same species and this will act as a reproductive barrier among the other related species.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.