Five closely related species of frogs live in ranges that overlap. some breed in woodland ponds, some in lowland swamps, and some in streams. how do these five species of frogs remain separate and do not attempt to mate? a line graph plots mating activity of 5 frog species over time, which ranges from march to july. the graph for each species is a bell-shaped curve. each curve overlaps with 1 or 2 neighboring curves. from left to right, the curves are labeled wood frog, leopard frog, pickerel frog, green frog, and bullfrog. study the graph and the text to identify two modes of isolation. a. temporal isolation: the species remain separate because the period of most active mating differs. b. behavioral isolation: each species has a courtship pattern that allows males and females to recognize each other. c. geographical isolation: the species remain separate because they have different breeding sites. d. reproductive isolation: the sperm of one species cannot fertilize the eggs from another species.

For your initial isolation of the bacterium, you simply needed to grow enough of it that you could identify some of its more general features. thus, you used a complex nutrient agar that would allow for the growth of almost any bacterial species. different bacterial species often have very specific requirements for life. this is a fact that you can utilize when you are trying to identify the bacterium to the species level. growth conditions that will permit the growth of one species but not another may allow you to select which one you would like to culture. you can utilize different selective/differential media that allow for the growth of only one type of bacteria (e.g., either gram positive or gram negative). culturing media such as phenylethyl alcohol or sodium chloride agar will be selective/differential for gram-positive bacteria. you may also want to show that it is different from other bacteria that may be present, so you could use a selective/differential agar. selective/differential media such as blood agar or phenol red sugar broths allow for the determination of whether a bacterial cell can grow and utilize specific ingredients included in the medium and turn the agar a distinct color. sheep's blood agar utilizes red blood cells to differentiate which bacterial species may have hemolytic properties.selective or differential? 1. an agar that uses a high salt concentration to limit the growth of one type of bacteria over another would be considered selective/differential. 2. if an agar contains a dye or ph indicator in the presence of a sugar, it is generally considered selective/differential for those bacteria that may ferment the sugar over those that cannot. 3. an agar that uses the dye in crystal violet agar suppresses the growth of gram-positive bacteria. this agar would be considered selective/differential. 4. the selective/differential aspect of macconkey agar allows for the determination of which bacteria are lactose fermenters and which are not. 5. a blood agar plate contains sheep red blood cells and allows for the determination of hemolytic capabilities for all bacteria that grow on the agar. this would make this type of agar selective/differential. 6. emb agar uses lactose and two dyes that allow it to be selective/differential between the blue-black colonies of e. coli and the pinkish colonies of all other enteric bacteria. 7. bile salts in macconkey agar allow for the agar to be selective/differential for the growth of enteric gram-negative bacteria over gram-positive bacteria. 8. an agar that allows for the distinction of bacteria based on metabolism would be considered selective/differential.

Which of these statements are true about scientific theories and hypothesis

The breakdown of food is accomplished by enzymes. a. physical b. chemical c. mechanical d. none of the above