Aresearcher has sequenced a gene that has two alleles. she has a mixture of both alleles and clones them into e. coli using plasmids. she is interested in one particular allele that is correlated with a disease. how can she select those colonies that have the disease allele?

The blue-white screen refers to a procedure of screening that allows for the brisk and convenient determination of recombinant bacteria in vector-based molecular cloning experiments. In the process, the DNA of interest is ligated into a vector. The blue colonies constitute of self-relegated plasmids that are devoid of DNA inserts obstructing the lac Z gene.  

The white colonies contain bacteria that conduct plasmids, which possess the inserts of DNA fragments that obstruct the lac Z gene. Therefore, the researcher needs to sequence the plasmids of the white colonies prior to knowing which allele it constitutes.  

image result for how many calories of energy will 20 grams of carbohydrates provide in one gram provides 4 calories

consuming the right balance of protein, carbohydrates and fat can you maintain a healthy weight and optimize your energy levels. protein and carbohydrates both contain 4 calories per gram, while fat provides 9 calories per gram.