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Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as β-lactamase), an enzyme present in some resistant bacteria. the mass of this enzyme in staphylococcus aureus is 29.6 kd. the amount of penicillin hydrolyzed in 1 minute in a 10-ml solution containing 10-9 g of purified penicillinase was measured as a function of the concentration of penicillin. assume that the concentration of penicillin does not change appreciably during the assay. penicillin(μm) amount hydrolyzed(nmol) 1 0.11 3 0.25 5 0.34 10 0.45 30 0.58 50 0.61 (a) plot v0 versus [s] and 1/v0 versus 1/[s] for these data. does penicillinase appear to obey michaelis-menten kinetics? if so, what is the value of km? (b) what is the value of vmax? (c) what is the turnover number of penicillinase under these experimental conditions? assume one active site per enzyme molecule.

Which of the following is not true of the formation of a bacterial translation initiation complex? a. if-3 binding to gtp is required for the charged trna to associate with the first mrna codon. b. n-formylmethionyl-trnafmet binds in the p site. c. if-1, if-2, and if-3 dissociate upon the 50s subunit binding. d. the 30s ribosome associates with the mrna before the 50s ribosome.

The picture depicts budding in the yeast cell. what is the result of this process? a) the bud develops into a yeast cell with new genes. b) the bud develops into a yeast cell with the same genes as the parent cell. c) the bud develops into a yeast cell with half the genetic information from the parent. eliminate d) the bud develops into a yeast cell with double the genetic information