Describe facilitated diffusion of ions through the channel proteins

Facilitated diffusion is usually significant to pass the ions across the hydrophobic layer of the plasma membrane. Transmembrane integral protein and careers proteins provide the channels that allow the ion to pass across the membrane.  When an ion bind to their active site on the protein (note that the proteins are very selective), the protein changes conformation. It is this change in conformation opens up the channel that allows the ions to be passed across the membrane. When the ions are released inside of the cell, the protein resumes normal shape (and the channel also closes) and the active site becomes available again or another ion.

littering streams           is the answer

this protocol describes a detailed procedure for intracellular flow cytometry staining.