Describe the relationship between crust , mantle ,lithosphere , asthenosphere, and tectonic plates.

Crust is the the upper layer of the earth. There are two types of crust:

1)Ocean crust

2)Continental crust

Tectonic plate are generally made up of earth crust. The upper layer is mantle.The mantle is the mostly-solid bulk of Earth's interior. .

Crust and the upper layer combined to form lithosphere.They are about 80Km  deep.

The asthenosphere is ductile . They are deformed like silicon polymer in response to the warmth of the earth. The rocks actually flows, when stressed is appiled they are start moving deep interior of earth.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.