Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of mrna and reverse transcriptase is part of a strategy to solve the problem of expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. the use of mrna and reverse transcriptase is part of a strategy to solve the problem of electroporation nucleic acid hybridization post-translational processing post-transcriptional processing restriction fragment ligation request answer