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Calculate the transformation efficiency. For: Sample 1:
Sample 2:
Sample 3:

Volume of calcium chloride: 250 μL
Volume of plasmid solution: 10 μL
Concentration of plasmid solution: 0.12
Volume of LB nutrient broth solution: 250 μL
Volume of transformation sample transferred to the agar plate: 100 μL

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Calculate the transformation efficiency. For: Sample 1:
Sample 2:
Sample 3:

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