Can someone calculate the transformation efficiency? Cos' I don't know what I am doin. T^T Volume of calcium chloride: 250 μL
Volume of plasmid solution: 10 μL
Concentration of plasmid solution: 0.12
Volume of LB nutrient broth solution: 250 μL
Volume of transformation sample transferred to the agar plate: 100 μL

renewable resources- resources that can be replenished (such as trees)

nonrenewable resources- resources that are gone once they are used (such as fossil fuels)

producer-person who makes goods or provides services

consumer- person whose wants are satisfied by using goods and services

allocate- distribute

choice- option

(hope this ! )

claude wants to buy a car and there are a few factors that will affect the decision he makes in choosing what car he should buy. the first factor that can affect his decision regarding what car to buy can be the social and the cultural influence of the society he is living in. for example, there might be a certain car that might have a better value in the eyes of the society or in other words a better social norm regarding that car.

another factor can be personal habits. claude might need a spacious car because he might have kids or might utilize the back seat for other purposes hence a sports car (2 seated) won’t be appropriate.  

stop doing work have fun

explanation:

answer:

the crusades were organized by western european christians after centuries of muslim wars of expansion. their primary objectives were to stop the expansion of muslim states, to reclaim for christianity the holy land in the middle east, and to recapture territories that had formerly been christian.

explanation:

you can literally google this.